This adapter will enable you to connect normal Amiga peripherals to an Amiga 1000. The Amiga 1000 has a male connector at the computer instead of a normal female connector. And some signals has changed places.
25 PIN D-SUB FEMALE to the Amiga 1000
25 PIN D-SUB FEMALE to the Amiga peripheral
All other straight over, 1 to 1, 2 to 2…